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Literature summary extracted from
- Functional importance of motif I of pseudouridine synthases: mutagenesis of aligned lysine and proline residues (2000), Biochemistry, 39, 9459-9465.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.99.25 | K19M | kcat is 8fold lower than wild-type value. KM-value is 11fold higher than wild-type value | Escherichia coli |
| 5.4.99.25 | K19R | kcat is 10fold lower than wild-type value. KM-value is 6fold higher than wild-type value | Escherichia coli |
| 5.4.99.25 | additional information | mutant enzymes with substitution of the nearly invariant lysine and proline residues in motif I of RluA display only very mild kinetic impairment. Substitution of the aligned lysine and proline residues reduces structural stability | Escherichia coli |
| 5.4.99.25 | P20G | kcat is 4.8fold lower than wild-type value. KM-value is 2.2fold higher than wild-type value | Escherichia coli |
| 5.4.99.25 | P20L | kcat is 2.1fold lower than wild-type value. KM-value is 3.5fold higher than wild-type value | Escherichia coli |
| 5.4.99.28 | P29G | kcat-value is 1.6fold lower than wild-type value. KM-value is 2.9fold higher than wild-type value | Escherichia coli |
| 5.4.99.28 | P29L | kcat-value is 1.9fold lower than wild-type value. KM-value is 4.2fold higher than wild-type value | Escherichia coli |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.25 | 0.000148 | - |
tRNA uridine55 | pH 7.5, 37°C, Escherichia coli tRNAPhe, wild-type enzyme | Escherichia coli | |
| 5.4.99.25 | 0.000324 | - |
tRNA uridine55 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P20G | Escherichia coli | |
| 5.4.99.25 | 0.000517 | - |
tRNA uridine55 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P20L | Escherichia coli | |
| 5.4.99.25 | 0.0009 | - |
tRNA uridine55 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme K19R | Escherichia coli | |
| 5.4.99.25 | 0.00161 | - |
tRNA uridine55 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme K19M | Escherichia coli | |
| 5.4.99.28 | 0.000108 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, wild-type enzyme | Escherichia coli | |
| 5.4.99.28 | 0.000318 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P29G | Escherichia coli | |
| 5.4.99.28 | 0.000455 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P29L | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.25 | Escherichia coli | P60340 | - |
- |
| 5.4.99.28 | Escherichia coli | P0AA37 | - |
- |
| 5.4.99.29 | Escherichia coli | P0AA37 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.25 | tRNA uridine55 | substrate: Escherichia coli tRNAPhe | Escherichia coli | tRNA pseudouridine552 | - |
? |  |
| 5.4.99.28 | tRNA uridine32 | - |
Escherichia coli | tRNA pseudouridine32 | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.25 | TruB | - |
Escherichia coli |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.25 | 37 | - |
assay at | Escherichia coli |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.25 | 36 | - |
Tm-value for mutant enzyme P20L is 35.9°C | Escherichia coli |
| 5.4.99.25 | 38 | - |
Tm-value for mutant enzyme P20G is 38.3°C | Escherichia coli |
| 5.4.99.25 | 40 | - |
Tm-value for wild-type enzyme is 40.2°C | Escherichia coli |
| 5.4.99.28 | 35 | - |
Tm-value for mutant enzyme P29L | Escherichia coli |
| 5.4.99.28 | 38 | - |
Tm-value for mutant enzyme P29G is 37.9°C | Escherichia coli |
| 5.4.99.28 | 45 | - |
Tm-value for wild-type enzyme is 44.9°C | Escherichia coli |
| 5.4.99.29 | 35 | - |
Tm-value for mutant enzyme P29L | Escherichia coli |
| 5.4.99.29 | 38 | - |
Tm-value for mutant enzyme P29G is 37.9°C | Escherichia coli |
| 5.4.99.29 | 45 | - |
Tm-value for wild-type enzyme is 44.9°C | Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.25 | 0.011 | - |
tRNA uridine55 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme K19R | Escherichia coli | |
| 5.4.99.25 | 0.012 | - |
tRNA uridine55 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme K19M | Escherichia coli | |
| 5.4.99.25 | 0.11 | - |
tRNA uridine55 | pH 7.5, 37°C, Escherichia coli tRNAPhe, wild-type enzyme | Escherichia coli | |
| 5.4.99.25 | 0.23 | - |
tRNA uridine55 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P20G | Escherichia coli | |
| 5.4.99.25 | 0.52 | - |
tRNA uridine55 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P20L | Escherichia coli | |
| 5.4.99.28 | 0.051 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P29L | Escherichia coli | |
| 5.4.99.28 | 0.061 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P29G | Escherichia coli | |
| 5.4.99.28 | 0.099 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, wild-type enzyme | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.25 | 7.5 | - |
assay at | Escherichia coli |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.25 | 7.43 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, wild-type enzyme | Escherichia coli | |
| 5.4.99.25 | 7.45 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme K19M | Escherichia coli | |
| 5.4.99.25 | 12.2 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme K19R | Escherichia coli | |
| 5.4.99.25 | 709.8 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P20G | Escherichia coli | |
| 5.4.99.25 | 1006 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P20L | Escherichia coli | |
| 5.4.99.28 | 112.1 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P29L | Escherichia coli | |
| 5.4.99.28 | 191.8 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P29G | Escherichia coli | |
| 5.4.99.28 | 916.7 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, wild-type enzyme | Escherichia coli |
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